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(73-03) Cardiovascular Toxicity of Propranolol in Rainbow Trout. Huggett, Duane*,1, Nichols, John2, Lien, Gregory2, Foran, Christy1,3, Schlenk, Daniel, 1 University of Mississippi, University, MS2 U.S. EPA, Duluth, MS3 Uiversity of California Riverside, Riverside, CA ABSTRACT- Propranolol is a beta-adrenergic receptor blocking pharmaceutical (B-blocker) that is used primarily to treat hypertension and angina. Recently, propranolol has been identifed in United States and European wastewaters. With a moderate Log P(3.67), propranolol has the potential to accumulate and cause target organ toxicity (ie cardiovascular toxicity) in fish. Preliminary experiments indicated that a bolus dose of propranolol >100ug/ml (>1.6 ug propranolol/ml blood) into the dorsal aorta would cause a decrease in heart rate but not arterial pressure. Rainbow trout were then exposed for 2hr to aqueous propranolol at concentrations of 46 and 460 ug/L. No changes in heart rate or arterial pressure were observed in fish expsoed to the low treatment level. Heart rate was significantly decreased after 1 hr of exposure to 460 ug propranolol/L. Diastolic pressure was observed after 1.5 hr of exposure to the high treatment level but, no changes in mean or systolic pressure were observed. Fish were then exposed to 460 ug propranolol/L with blood samples collected from the dorsal aorta. Effects levels corresponded to a propranolol plasma concentration of >100 mg/ml. Key words: pharmaceuticals, cardiovascular , trout |
