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(18-18) Combined in situ and in vitro experiments to assess estrogenic activity of environmental water samples . Pawlowski, S.*,1, Ternes, T.2, Rastall, A.3, Erdinger, L.3, Braunbeck, T.1, 1 Zoological Institute I, University of Heidelberg, Heidelberg, Germany2 ESWE Stadtwerke Wiesbaden, Wiesbaden, Germany3 Hygiene Institute, University of Heidelberg, Heidelberg, Germany ABSTRACT- Estrogenically active compounds could be indentified in several waste water effluents and river water using chemical analyses across Germany. However, biological data concerning estrogenic effects in fish and fish cell cultures at the respective regions have not been adequately appreciated. In the present study, vitellogenin induction in male and juvenile rainbow trout (Oncorhynchus mykiss) exposed to sewage effluents and river water were repeatedly investigated after a two week exposure over a period of one year. Whilst fish were exposed to the respective effluents or river waters, water samples were taken, extracted and used for in vitro assessement of estrogenic activity in primary rainbow trout hepatoyctes and the yeast estrogen screen. Furthermore, chemical analyses of water samples were performed. Finally, data from the in situ experiments and the two in vitro experiments were analysed with respect to possible similarities. In most cases, data resulting from in vitro experiments could, indeed, be correlated with the data from the corresponding in situ experiments with the rainbow trout. Key words: endocrine disruptor, rainbow trout, primary hepatocytes, yeast estrogen screen |
