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(38-10) Predicting uptake and bioavailability of organic contaminants in terrestrial organisms using SPME in different soils. van der Wal, Leon*,1,4, Jager, Tjalling2, Fleuren, Roel3, van Gestel, Cornelis4, Hermens, Joop1, 1 Institute for Risk Assessment Sciences (IRAS), Utrecht University, P.O. Box 80176, Utrecht, the Netherlands4 Institute of Ecological Science, Vrije Universiteit, de Boelelaan 1087, Amsterdam, the Netherlands2 Department of Theoretical Biology, Vrije Universiteit, de Boelelaan 1087, Amsterdam, the Netherlands3 National Institute of Public Health and the Environment (RIVM), Laboratory for Ecotoxicology, P.O Box 1, Bilthoven, the Netherlands ABSTRACT- The influence of "bioavailability" on the effects of soil pollution is of great importance in determining adverse effects in contaminated areas. Different extraction techniques (solvent mixtures, C18-coated membrane disk) have been applied in order to take into account bioavailability in the prediction of internal concentrations in terrestrial organisms. Our work focusses on the applicability of Solid Phase Micro Extraction (SPME) as a tool to estimate bioavailability and internal concentrations of contaminants in terrestrial organisms such as enchytraeids and earthworms. Earlier results indicated the applicability of SPME fibers for this purpose in a natural soil for chemicals with a log Kow up to 6. These results were confirmed using artifical OECD soil spiked with chlorobenzenes in the same range of log Kow. Recent work focusses on the uptake of other chemicals (PAHs, PCBs, pesticides) with a wider range of log Kow in a natural soil amended with dredge sludge material. Uptake of contaminants into both SPME fibers and biota exposed to this natural soil were measured until equilibrium was reached. The uptake into both biota and fibers is compared. Key words: SPME, bioavailability, soil, terrestrial organisms |
