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(18-21) Evaluation of the estrogenic activity of molecules present in cosmetics (parabens, UV-screens and musk fragrances). Casellas, Claude*,1, Gomez, Elena1, Pillon, Arnaud2, Fenet, Hélène3, Boussioux, Anne-Marie2, Bintein, Sylvain4, Nicolas, Jean-Claude2, Balaguer, Patrick2, 1 DSESP, Faculté de Pharmacie, Montpellier, France2 INSERM 439, Montpellier, France3 Laboratoire de Chimie Analytique, Faculté de Pharmacie, Montpellier, France4 Ministère de l'Aménagement du Territoire et de l'Environnement, Paris, France ABSTRACT- A great number of molecules are present in the formulation of pharmaceuticals, personal care products, laundry detergents and food additives. Their wide range of applications multiply the possible routes of exposure for human and for the environment; some of them have shown different degrees of estrogenic activity. Parabens are an antimicrobial agents in foods, drugs, cosmetics and toiletries found in more than 13,200 formulations. Ultraviolet (UV) screens are used for the protection about UV radiation and as additives for light stability. Synthetic musk fragrances are found in perfumes, cosmetics and laundry detergents (production about 7000 t/year). Studies of estrogenicity of parabens reported contradictory results in in vivo and in vitro tests, several UV-screens possess estrogenic activity and nitromusks interacts with the estrogen receptor of fishes. Our objective is to determine the estrogenic activity of some conservatives, UV-screens and perfumes on stably transfected MELN cell lines. The cell lines were obtained by stable transfection of the breast cancer MCF7 cells and enable the detection of estrogenic activity by measuring the luciferase transactivation. The molecules tested were p-hydroxybenzoic acid and their methyl-, ethyl-, propyl- and butyl- esters, the UV-screens Benzophenone-3, Homosalate, 4-methyl-benzylidenecamphor, octyl-methoxycinnamate and octyl-dimethyl-PABA, and the fragrances musk ketone, musk moskene, Galaxolide, Celestolide and Cashmeran. The blockage with the antiestrogen ICI was used for the confirmation of ER mediated activities. The evaluation of the estrogenic potency of these molecules allows a direct comparison of their potential hazard in an in vitro sensitive, selective cell lines for an environmental monitoring. Key words: estrogenic activity, cosmetics, reporter cell lines, in vitro assays |
