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(70-51) Toxicokinetic of heavy metals after the Aznalcollar Mining Spill using Ruditapes philippinarum. Martín-Díaz, M. Laura1, Blasco, Julián2, González de Canales, M. Luisa3, Sales, Diego4, DelValls, Angel*,1, 1 Departamento de Química Física. Facultad de Ciencias del Mar y Ambientales. Campus río San Pedro s/n, Puerto Real. Cádiz, Spain2 Instituto de Ciencias Marinas de Andalucía. Consejo Superior de Investigaciones Científicas (CSIC). Campus Río San Pedro s/n., Puerto Real. Cádiz, Spain3 Departamento de Biología. Facultad de Ciencias del Mar y Ambientales. Campus río San Pedro s/n, Puerto Real. Cádiz, Spain4 Departamento de Tecnología del Medio Ambiente. Facultad de Ciencias del Mar y Ambientales. Campus río San Pedro s/n, Puerto Real. Cádiz, Spain ABSTRACT- The Guadalquivir River is the main river of the South of the Iberian Peninsula and shelters different kind of species. On the 25th April 1998, the giant holding pool of the Aznalcollar mine owned by the Canadian-Swedish Company Boliden Ltd. burst its banks. The mining accident produced almost 6 Hm3 of mud and acidic waters, with high concentrations of metals in solution including, Cd, Cu, Mn, As, Pb and especially Zn. This spill poured into the nearby Guadiamar River, which is a tributary of the Guadalquivir River that flows to the Guadalquivir Estuary the ecosystems from these aquatic environments were negatively impacted. The early impact of the spill on the ecosystem has been previously reported by (Gomez-Parra, et al., 2000). The authors report the heavy metal concentrations in the mentioned ecosystem. A set of the previously reported environmental concentrations was selected to determine the adverse effect on the clam Ruditapes philippinarum that was selected as bioindicator. Individuals of this organism were exposed to the mentioned heavy metal concentrations to simulate the early effect on the system. Animals were exposed, during 21 days, to two different concentrations of zinc, copper and cadmium, 700 mgL-1 and 340 mgL-1, 7.5 mgL-1 and 15 mgL-1, 1.20 mgL-1 and 3 mgL-1 respectively. Effect of these concentrations at two different salinities, 10 and 35, was measured through the analysis of bioaccumulation in gills, metallothioneins in midgut, and histology in gills and midgut. Clams were dissected every seven days, and samples were taken an analyzed. Results show differences between treatment and the control. Key words: biomarkers, histopathology, simulation experiments, bioaccumulation |
