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(58-06) Integrated biological effect monitoring. Broeg, Katja*,1, Zander, Susanne2, Krüner, Günther1, Körting, Wolfgang2, v. Westernhagen, Hein1, 1 Alfred-Wegener-Institute for Polar and Marine Research, Bremerhaven, Germany-2 School of Veterinary Medicine, Hannover, Germany- ABSTRACT- A five year study on flounder (Platichthys flesus) was aimed in forging links between well known biomarkers at different levels of biological organisation and predicting effects on the ecosystem level in the German Bight. The applied biomarkers were: 1. Induction of CYP1A1 EROD as a biomarker of exposure at the molecular level. 2. Membrane stability of hepatocyte lysosomes as a biomarker of toxic effect at the subcellular level. 3. Changes of the activity of non specific immune response in the liver as a biomarker of toxic effect at the cellular level. 4. Accumulation of neutral lipids in flounder liver as a biomarker of toxic effect at the organ level. 5. Parasite species richness as a biomarker of toxic effect at the community level. Based upon the results obtained from the examination of 630 individual fish and additional residue analyses, a time scale of biomarker response was developed. Key events can be distinguished for four steps leading from "no response" in healthy fish, to "early response" at the onset of disturbance due to anthropogenic pollution and "medium late" response and "late response" in fish heavily impaired. Key words: integrated biological effect monitoring, biomarker, Platichthys flesus, pollution |
