|
(18-74) Standardized amphibian maintenance-food. Kashiwagi, Akihiko*,1, Nakahata, Akiko2, Kashiwagi, Keiko1, Tooi, Osamu2, Arizono, Koji3, 1 Laboratory for Amphibian Biology, Graduate School of Science, Hiroshima University, Hiroshima, Japan2 Towa Kagaku Corporation. Ltd., Hiroshima, Japan3 Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Kumamoto, Japan ABSTRACT- To investigate the possible effects of food on developing tadpoles. TK stage I tadpoles of Rana rugosa and NF stage 45 tadpoles of Xenopus laevis were fed on boiled spinach (group 1), Sera micron (group 2), medaka's food containing a small amount of estrogen (group 3), and medaka's food plus choresterol (group 4). Results showed : (1) R. rugosa group 1 tadpoles were normal in development, and completed metamorphosis. Group 2 tadpoles were of various sizes, preyed on each other during early larval stages, and 50% completed metamorphosis. 70% of group 3 and 4 tadpoles died, and the remaining 30% completed metamorphosis. (2) For X. laevis still under investigation, group 2 tadpoles are normal, and 50% completed metamorphosis. Group 1 tadpoles are at stages 49-57, only one completing metamorphosis. Group 3 and 4 tadpoles began to appear from day 90 on, but were underdeveloped, and subsequently died. Key words: Tadpole, Food, Metamorphosis, Development |
