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(72-03) Benzene induced chromosome aberrations and the association with genetic polymorphism. kim, Su Young*,1, Cho, Yoon Hee1, Jung, Eun Jung1, Lee, Young Joon1, Choi, Jung keun2, Paek, Do Myung1, Chung, Hai Won1, 1 School of Public Health, Seoul National University, Yun Keun-dong 28, Chong ro-ku, Seoul, Korea2 Occupational Safety & Health Research Institute, Korea Occupational Safety & Health Agency, Inchon, Korea ABSTRACT- This study was done to examine the benzene induced chromosomal aberrations and also the influence of genetic polymorphism(GSTM1, GSTT1, GSTP1, NAT2, NQO1, CYP2E1 and CYP1A1) on the chromosomal aberrations. In total, 82 benezene exposed workers and 76 matched controls were examined. The frequencies of chromosome aberrations including aneuploidy and structural aberrations were increased in benzene exposed workers compared to unexposed control. The aneuploidy for chromosome 8 or 21 were increased in benzene exposed workers. The frequency of translocation between chromosome 8 and 21[t(8:21)] was also increased in benzene exposed workers. Multiple regression analysis was done to find the association between various type of chromosomal aberrations and genetic polymorphism of metabolizing enzymes. GSTM1 and GSTT1 null genotype is associated with the elevated frequencies of aneuploidy, but not with the frequencies of structural aberrations. Individuals with NAT2 slow acetylator genotypes showed increased frequencies of trisomy for chromosome 8. The variant types of NQO1 genotype was also associated with the increased frequencies of translocation between chromosome 8 and 21. While CYP1A1 or CYP2E1 alone showed no associations, when analyzed together with genes for detoxifying enzymes such as GSTM1, T1, GSTP1, or NQO1, the combined genotypes were significantly associated with the increased frequencies of chromosomal aberration. In conclusion, the influence of genetic polymorphism of xenobiotic-metabolizing enzymes on the susceptibility of individuals to the induction of chromosomal aberration by benzene was suggested. Key words: benzene, aneuploidy, genetic polymorphism , chromosomal aberrations |
