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(80-64) ISIS-Oxidative stress induced in EPC-cells by sediment extracts from North Sea and Baltic Sea. Sierts-Herrmann, Arne*,1, Akkaya, Ferhat1, Kinder, Angelika1, Steinhart, Hans1, 1 University of Hamburg, Hamburg, Germany ABSTRACT- Oxidative stress, caused by reactive oxidative species, is one of many possible effects induced by anthropogenic stressors in the marine ecosystem and results in grave impairments of cellular rank like enzyme activity inhibition, DNA-damage up to cell death. A chemical-induced oxidative stress of sediment extracts in aquatic organisms is documented in several studies. Primarily, these investigations are focussed on relative high polluted sites (i.e. industrial regions), but only few is known about the anthropogenic effects of marine sediments from North Sea and Baltic. Within the scope of the "ISIS"-project (identification of sediment bound contaminants from North Sea and Baltic Sea) the induction of oxidised glutathione (GSSG) in a EPC-cell line (Epithelioma papulosum cyprini) is used as an appropriate marker for oxidative stress. To encircle the causing agents of the observed effects the approach of bioassay-directed fractionation was chosen. Significant effects for the crude extracts and for three steps of fractionation (gel permeation chromatography, and high performance liquid chromatography) were detectable, in the course of which the intensities decreased. Nevertheless, the effects in the subsequent fractions point to a great spectrum of oxidative stressors over a wide range of polarity. Several positive controls were tested to evaluate potential inducers of oxidative stress for the used EPC-cells. Mainly redox-cycling quinones like menadione and 1,2-naphthoquinone or direct thioloxidans like diamide, but also sulfur and copper-(II)-sulfate act as strong oxidative stressors. In comparison, the effects of compounds with known release of oxidative stress (polycyclic aromatic hydrocarbons, nitroaromatic compounds, bipyridiles like paraquat and pesticides like dieldrine and lindane) were negligible. Key words: oxidative stress, glutathione, EPC-cells, sediment |
