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(71-03) Functional and structural impact of Linuron on different primary producers. Slijkerman, Diana*,1,2, dos Santos, Matilde3, Soares, Amadeo4, Ribeiro, Rui3, Jak, Robbert1, 1 TNO Institute of Environment, Energy and Process Innovation, Den Helder, The Netherlands2 Institute of Ecological Science, Vrije Universiteit, Amsterdam, The Netherlands3 Instituto do Ambiente e Vida, Universidade de Coimbra, Coimbra, Portugal4 Campus Universitario de Santiago, Aveiro, Portugal ABSTRACT- Within the TARGET project in situ bioassays are being developed in order to assess the functional status of water systems. The new test methods support the objectives of the European Water Framework Directive, and complement existing structural ecological measures of water quality. To test and validate the new developed bioassays mesocosm studies are carried out with different types of toxic substances. To determine the specific abilities of the bioassays focussing on the functional process of primary production the herbicide Linuron was used during one of these mesocosm studies. A total of 9 outdoor mesocosms was used, three replicates for the control and two replicates for each dosed system. Concentrations used during the study were 20, 60 and 180 Key words: mesocosm, functionality, herbicide, algal beads |
g/L Linuron, active ingredient. The algal bead growth inhibition bioassay was deployed to study the growth of immobilized cells of the algae Chlorella vulgaris during the first 21 days of the study. In addition, the growth of the macrophyte Elodea nuttallii was studied; and phytoplankton species composition and total chlorophyll a content in the water column determined at different time intervals. A comparison between the response of the functional in situ bioassay, and the functional and structural end points derived from this mesocosm study will be presented.