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PARENT SESSION
41 - Physiological and Molecular Mechanisms of Toxicity
8:00 AM to 6:30 PM, Tuesday, 14 May 2002
Exhibition Area

(41-12) Histopathological effects of surfactants in the clams Ruditapes philippinarum.

Sáez, Mónica1, Ortiz, Juan Bosco1,2, Gómez-Parra, Abelardo1, Sarasquete, Carmen2, González-Mazo, Eduardo*,1, 1 Facultad de Ciencias del Mar. Universidad de Cádiz, Puerto Real, Cádiz2 Instituto de Ciencias Marinas de Andalucía. CSIC, Puerto Real, Cádiz

ABSTRACT- Surfactants show high affinity by the interfaces among which are included the biological membranes whose constitute an interface between organism and the medium. The clam Ruditapes philippinarum has been exposed to anionic (LAS) and nonionic (NP and NPEO) surfactants in a flow-through system. The bioassay consists on a exposure and a depuration phase and the specimens were collected at the beginning of the exposure period and also at the end of both, exposure and depuration periods. The organs studied were the gills and the digestive gland.Histopathological alterations were observed in clam specimens treated with surfactants. Pathological alterations increase, in general, with concentration and time of exposure of contaminants. Digestive gland (hepatopancreas) and gills shown diverse cellular alterations (hyperplasia and tissue disorganization). In the LAS case, in detoxified specimens, digestive gland showed an evident epithelial regenerative process.

Key words: histopathology, surfactants, Ruditapes philippinarum