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(22-25) Evaluation of different subcellular and cellular test systems for an ecotoxicological risk analysis. Stock, Frauke*,1, Jastorff, Bernd1, 1 UFT, Center for Environmental Research and Technology, Leobener Str., Bremen, Germany ABSTRACT- In addition to an increasingly complex testing of environmental effects of chemicals, for example by means of mesocosms, the study of the mechanisms of these effects gained importance during the last years. Chemicals are investigated in retrospective using toxicological tests, particular methods from molecular biology. But mechanistical studies can also be of great advantage for prospective risk analysis: The application is fast and cost-effective and therefore the tests can be used within the first stages of product development to estimate potential impacts on organisms. Structure activity relationships can be proposed and verified and the results can be useful for a new sustainable product-design. Because these tests investigate principle mechanisms which can occur in nearly all organisms they avoid dealing with the varying sensitivity of different organisms. To use these advantages a well-considered selection of potential end points is necessary. Additionally the results need to be incorporated into a risk analysis concept which includes other important information about the chemicals such as bioavailability and bioaccumulation. Different toxicological tests are evaluated during a doctoral thesis concerning their suitability for the ecotoxicological risk analysis. The main focus is to study endpoints connected to signal transduction and detoxification, either at a cellular or subcellular level. The significance of the tests is discussed and their possible integration into existing risk analysis procedures are shown. First experiences with some of the tests, as well as some results for antifouling biocides, will be presented. Key words: subcellular tests, cellular tests, risk analyis, antifouling biocides |
