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(70-65) Assessing the influence of biota in the mobility of pollutants within the Multi-Species Soil Systems (MS 3). Alonso, Elena*,1, Fernandez, Carlos1, Najera, Isaac, Garcia, Pilar1, Heranz, Paloma1, Vascuas, Esther1, Tarazona, Jose1, Carbonell, Gregoria1, 1 Laboratory for Ecotoxicology, INIA, Madrid, SPAIN ABSTRACT- The MS 3 (Multi-Species Soil System) is an experimental approach developed by the Laboratory of Ecotoxicology of INIA to investigate simultaneously the fate and effects of pollutants on soil systems. Several experimental designs have been developed. The MS 3 TOX-FATE are used to assess the effects and mobility of pollutants and their degradation products in the soil. The presence of the organisms can influence the mobility of the pollutants within the soil column. This influence is assessed in this experiment. To achieve this goal, three different concentrations of Cu and Cd (1/60, 1/24 and 1/12 of the 14 d LC50 for E. fetida) were studied on columns with and without organisms. After 25 days of simulated rainfall, the soil-cores were cut in seven profiles and the total and bioavailable concentration of metals were measured. Cu and Cd in the leachates (collected periodically) and organisms were also analized. Mobility of Cu and Cd was incresed due to the presence of organisms at low concentration (doses 1 y 2), the metals were distribute through the top 20-30 cm soil layer , although no significative differences in the bottom profile and leachates were found . At the highest concentration (strongly affecting earthworms and plants) metals remained in the two uppermost profiles, like in the columns without organism. Results confirm the influence of organisms in the distribution of pollutants within the top soil core, however metals were retained with the system, and no changes in the leachates were observed, thus this experimental approach supposes a high tier toxicity test. Key words: MS3, soil pollutant mobility, Cu, Cd |
