PARENT SESSION
80 - Biomonitoring and Assessment
8:00 AM to 6:30 PM, Wednesday, 15 May 2002
Exhibition Area

(80-11) ICES/IOC workshop on biological effects of contaminants in pelagic ecosystems (BECPELAG): in vitro bioassays.

Thomas, Kevin^*,1, Becker, Gerd², Hylland, Ketil³, Klungsøyr, Jarle⁴, Lang, Thomas⁵, McIntosh, Alistair⁶, Serigstad, Bjørn⁹, Thain, John¹, Vethaak, Dick⁷, Wosniok, Werner⁸, ¹ CEFAS, Burnham on Crouch, UK² BSH, Hamburg, Germany³ NIVA, Oslo, Norway⁴ IMR, Bergen, Norway⁵ BFA-Fi, Cuxhaven, Germany⁶ FRS, Aberdeen, UK⁹ Ocean Climate, Bergen, Norway⁷ RIKZ, Middleburg, Netherlands⁸ University of Bremen, Bremen, Germany

ABSTRACT- The ICES biological effects monitoring in pelagic ecosystems (BECPELAG) workshop is a multi-national, multi-dicipline workshop aimed at establishing suitable techniques for monitoring the effects of contaminants on pelagic ecosystems. One of the many activities that have been concurrently performed is the extraction of water samples using SPMDs, blue mussels and large volume SPE followed by in vitro testing and targeted chemical analysis of the concentrated extracts. SPMDs were deployed at four sites in the German Bight and four sites in a transect from an oil plafrorm in the North Sea. Water samples for extraction were taken in the same eight locations. The following in vitro assays will be used: AChE inhibition, fish hepatocytes, DR-CALUX, ER-CALUX, AR-CALUX, Microtox, Mutatox, yeast oestrogen and androgen screen (YES & YAS), Danio rerio, Corophium volutator, Oyster (Crassostrea gigas) embryo, Tisbe battagliai, Skeletonema costatum, yolk-sack turbot (Cophtalmus maximus) larvae, Arbacia punctulata, Arcartia tonsa, and Nitocra spinipes. This presentation describes the objectives of the study and preliminary results. More information can be found at the web-site for the workshop: http://www.niva.no/pelagic/web/.

Key words: BECPELAG, bioassays, pelagic ecosystems, extraction