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(56-10) Elimination of Bisphenol A in sewage treatment plants and it`s estrogenic capacity . Schaat, Annette1, Weltin, Diethelm2, Stahlschmidt-Allner, Petra1, Allner, Bernhard*,1, 1 Hessian Agency for the Environment and Geology, Wiesbaden, Germany2 Technische Universität Dresden, Institute of Waste Management, Pirna, Germany ABSTRACT- Experiments with a coupled unit OECD 302 biodegradation test system showed that influent concentrations of 1mg/L Bisphenol A (BPA) were reduced to concentrations < 200ng/L in the effluent after biological treatment. These data confirm an approximately complete elimination of the compound in course of sewage treatment. Estrogenic effects of BPA on zebrafish (Danio rerio) were estimated by a vitellogenin assay. Aprotinin stabilised serum proteins were separated by SDS-PAGE. An antiserum to highly purified vitellogenin of carp (Cyprinus caprio) has been used for immunological identification of the vitellogenin in Western blot. Flow through exposure of male zebrafish to the effluent of 1mg/L BPA spiked coupled unit test systems during the steady state phase of biodegradation for a 25 d period caused a weak induction of vitellogenesis. In order to check whether the estrogenic effects of the effluent are related to BPA degradation products or to the low concentrations of the parent compound, exposure tests with BPA were carried out. Semistatic exposure to 400 ng/L BPA (measured concentrations) for seven days also induced vitellogenesis in male zebrafish. Due to the fact, that effective concentrations of BPA are in the same range as BPA concentrations measured in German sewage treatment plant effluents, biodegradability of low concentrations of BPA in surface waters as well as estrogenic capacity of the compound should be investigated more detailed. Key words: Bisphenol A, sewage treatment, Vitellogenin, Zebrafish |
