|
(80-74) ISIS - PAHs, polycyclic musks and nitroaromatic compounds in sediments of the North Sea and Baltic Sea. Huehnerfuss, Heinrich1, Franke, Stefan1, Heinzel, Nicolas1, Francke, Wittko1, Theobald, Norbert2, Biselli, Scarlett2, Reineke, Ninja*,1, 1 University of Hamburg, Hamburg, Germany2 Federal Maritime and Hydrographic Agency, Hamburg, Germany ABSTRACT- The aim of the interdisciplinary project ISIS ( identification of sediment bound contaminants from North Sea and Baltic) is to identify ecotoxicologically relevant substances with the help of bioassay-directed chemical analyses. The focus was laid on organic pollutants in marine sediment extracts from the North Sea and Baltic Sea. In addition to the application of biotests and non-target-analyses for the identification, the concentrations of different classes of contaminants were determined and will be presented. The experimental method included extraction of wet sediment with ultra-turrax followed by a fractionation procedure with a combination of GPC and HPLC. The resulting fractions were investigated with different bioassays as well as with GC-MS analysis. Among well-known contaminants like PAHs and organochlorine pesticides, new class of xenobiotics such as polycyclic musks, triazines and several nitroaromatic compounds were included in the target analyses. The quantification procedure is quite difficult due to the high biogenic matrix and comparably low concentrations of most analytes. While the concentrations of PAHs were found in the medium ng-range (per g dw), the concentrations of HHCB (Galaxolide®), AHTN (Tonalide®) and the metabolite HHCB-lactone were generally two orders of magnitude lower. Triazines like terbutylazine could not be determined in the North Sea sediments investigated in this study. This had been anticipated because of their water solubility. Of specific interest are the chlorinated phosphates tris-(2-chlorethyl)phosphate (TCEP) and tris-(1-chlorpropyl)phosphate (TCPP) that obviously have a strong capacity to bind to particles despite their high polarity. So far there is little knowledge about the effects of these anthropogenic organic pollutants in sediments. The simultaneous determination of the ecotoxicological effects in the same fractions may be helpful for a correlation. Further investigations with regard to the ecotoxicity of those substances are still taking place within the cooperation of the other research groups of the ISIS project. Key words: bioassay-directed chemical analysis, sediment contamination, PAHs, polycyclic musks |
