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(47-06) Dutch survey of chlorinated PBTs in industrial effluents and other emission sources. Van Hattum, Bert*,1, Lamoree, Marja1, Senhorst, Henk2, Sanderson, Thomas3, 1 Institute for Environmental Studies, Amsterdam, Netherlands2 Institute for Inland Water Management and Waste Water Treatment, Lelystad, Netherlands3 Institute for Risk Assessment Sciences, Utrecht, Netherlands ABSTRACT- The first results will be presented from a national survey (OVOC) on the presence of chlorinated PBTs (persistent, bioaccumulative and toxic compounds) in (19) effluents from industrial and municipal waste water treatment plants. The project is sponsored snd supervised by the Dutch government (VROM, VW), chemical industry (VNCI) and national NGOs. A bioassay-directed approach was used, with sensitive in vitro screening methods for compounds with dioxin-like (Ah receptor-responsive reporter system DR-Calux and EROD induction in carp hepatocyte) or estrogenic (vitellogenin induction in carp hepatocytes) mode of action, in vivo toxicity testing (zebrafish-early life stage mortality, Microtox), C-18 fractionation, and chemical screening (GC-ECD/MSD). DR-Calux, EROD activity in carp-hepatocyte and the semi-chronic Microtox-assay were responsive to various samples; estrogenic compounds were not detected. Halogenated compounds were detected in fractions with different hydrophobicity (Log Kow ranges low <4, medium 4-6 and high >6). Bioassay-based dioxin equivalents (TEQ), as determined with the DR-CALUX and EROD assays were higher than indicated by chemical monitoring of PCDDs and PCDFs. A selection of samples has been subjected to biodegradation and toxicity identity evaluation studies (TIE) to identify bioactive compounds. In addition to the effluents various solvents and commonly marketed products, as well as atmospheric emission sources will be included in the sceeening study. The approach chosen allows a classification and estimation of the significance of PBT emissions in the Netherlands. Key words: bioassay-based screening, TIE, PBTs, wastewater |
