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(70-77) Acute and Chronic Toxicity of Nicekl to Rainbow Trout. Brix, Kevin*,1, DeForest, David2, Keithly, James2, Laughlin, Jim2, Ortego, Lisa3, 1 EcoTox, North Bend, Washington2 Parametrix, Inc., Kirkland, Washington3 NiPERA, Durham, North Carolina ABSTRACT- Nickel toxicity to aquatic life has not received the same amount of attention by researchers as many other divalent essential metals (e.g., copper, zinc). Of the fish species tested in chronic exposures, rainbow trout (Oncorhynchus mykiss) is the most sensitive. In order to develop additional nickel toxicity data and study the dose-response relationship for rainbow trout, we conducted acute (96 hours) and chronic (75-day early life stage) flow-through nickel toxicity studies using the rainbow trout. In addition to the standard toxicological endpoints, we also measured nickel accumulation in the whole egg and larval fish gill over time in order to develop a tissue-residue based dose-response relationship (e.g., gill LA50). Additionally, we measured Na+, Ca++, and Mg++ concentrations in whole egg and larval blood at multiple time periods in order to explore the mechanism of acute and chronic nickel toxicity. Key words: Oncorhynchus mykiss, nickel, ionoregulation, biotic ligand model |
