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(86-01) Detailed review paper for fish reproductive screening assays. Blanton, Michael*,1, Schultz, Irv1, Nagler, Jim2, Touart, Les3, 1 Battelle Pacific Northwest Division, Sequim, Washington, USA2 University of Idaho, Moscow, Idaho, USA3 U.S. Environmental Protection Agency, Washington, D.C., USA ABSTRACT- A Detailed Review Paper (DRP) for a fish reproductive screening assay with endocrine endpoints is being developed by the U.S. Environmental Protection Agency. The fish screening DRP will summarize, explain, and document relevant principles, methods, and techniques recommended for development of an initial protocol(s), and will identify those issues that may require prevalidation studies to adequately address. The focus of the DRP will be on the relevance and sensitivity of the candidate assays/endpoints for identifying chemicals that affect the endocrine system in fathead minnows, zebrafish, and Japanese medaka. The DRP will provide a basis for selecting the most promising methods for assessing chemical effects on endocrine systems in fish and for determining future studies required to resolve outstanding issues relative to method development, including the need for additional targeted prevalidation studies. This information will be used to support the design of a draft protocol for use in the validation phase of protocol design, with the ultimate goal of developing future EPA/OECD test method guidelines. The preliminary findings from initial DRP efforts will be presented. Key words: endocrine disruption, detailed review paper, method, fish reproductive screening assay |
