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(40-02) Screening for distribution patterns of veterinary antibiotics in the environment. Christian, Thorsten1, Meyer, Michael2, Schneider, Rudolf*,1, Goldbach, Heiner1, 1 Institute of Agricultural Chemistry, Bonn, Germany, Germany2 United States Geological Survey, Ocala, Florida ABSTRACT- Pharmaceuticals have been showing up in surface waters in the United States as well as in Europe. Meanwhile it has become evident, that sewage treatment plants are important sources for this pollution as with a series of compounds they are not able to eliminate them from waste water from private households and hospitals effectively. Some of the antibiotic drugs on the other hand are not used in human medicine but for veterinary purposes. It is our goal to elucidate input pathways for this group of compounds. We have developed a screening procedure that integrates pre-screening of manure and water by immunoassay (ELISA) with multi-compound methods like HPLC/UV and LC/MS-MS. Water samples that proved positive in the screening are extracted by a solid-phase extraction procedure. Soils are extracted by Accelerated Solvent Extraction (ASE), as is manure after solidifying it with diatomaceous earth ("hydromatrix"). With this screening procedure we follow contaminations of surface water back to its probable origins, i.e. the adjacent fields and the manure that has been applied to them. Key words: immunoassay, water, soil, manure |
