|
(22-03) Seasonal biomarker variations relative to physiological indicators and chemical contaminant levels in Mediterranean mussels (Mytilus galloprovincialis). Bodin, N.*,1, Stanisière, J.2, Miralles, F.2, Chavanon, F.2, Ravel, C.2, Menard, D.1, Budzinski, H.3, Pfohl Leskowicz, A.4, Amat, A.4, Cherel, Y.5, Minier, C.6, Bocquené, G.1, Burgeot, T.1, 1 Ifremer, Nantes, France2 Ifremer, La Seine sur Mer, France3 LPTC University of Bordeaux, Talence, France4 ENSAT, Auzeville-Tolosane, France5 ENV, Nantes, France6 University of Le Havre, Le Havre, France ABSTRACT- Interpretation of the biological effects of chemical contaminants in a biomonitoring context requires good knowledge of the typology of study sites and the physiology of the model species. The purpose of this study, conducted over a two-year period on mussels (Mytilus galloprovincialis) transplanted into two experimental sites in the Mediterranean, was to validate several exposure biomarkers relative to variations caused by the natural environment. The mussels were placed in cages at two sites with different typologies: the Gulf of Fos sur Mer, which is subjected to heavy industrial and petrochemical pollution; and Fourcade, located near an urban area and a fish farm. Seasonal variations in the two mussel cohorts were monitored from April 1999 to April 2001. Biomarkers of early exposure (AChE, BPH, DNA adducts, MXR) and histological determinants useful for the characterisation of effects at longer term were measured monthly. Concentrations of chemical contaminants, such as PAH, PCB and heavy metals, were determined monthly in the same tissues of sampled mussels. Concurrently, several physico-chemical parameters (temperature, salinity, turbidity) and physiological indicators (condition indices, sex, gonad maturation) were analysed. An initial integrated interpretation of all these parameters was performed to identify the specific trends observable at the two experimental sites. Key words: biomonitoring, Mytilus galloprovincialis, biomarkers, seasonal variations |
