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(18-41) Analysis of steroids in biological organisms. Budzinski, Hélène*,1, Togola, Anne1, Labadie, Pierre1, Devier, Marie-Hélène1, Augagneur, Sylvie1, 1 LPTC-UNIVERSITE BORDEAUX I, TALENCE, GIRONDE, FRANCE ABSTRACT- The scientific community has demonstrated since several years that various chemical contaminants could influence human and animal endocrine systems. The list of these compounds still increases. We find the principal chemical contaminants (PAHs, PCBs, Pesticides, Phtalates, Metals, Alkylphenols, Dioxines), synthetic steroids and natural oestrogens. If we consider the important diversity of compounds that can be potentially classified as xenoestrogenic compounds, it appears important to study the hormonal metabolism of biological organisms and the endocrine disruption phenomena. The work presented here deals with the development of a methodology to study and quantify steroids in biological organisms (plasma and tissues). Various steps of extraction (SPE extraction) and purification (SPE purification) have been developed. The final identification and quantification is performed by GC/MS. This methodology enables the study of the testosterone metabolism in aquatic biological organisms. These developments have been applied to the study of hormonal metabolism in mussels in the Arcachon Bay (Atlantic South -West coast of France) and in fishes from Mediterranean Sea (collected in locations of different degrees of pollution). Key words: STEROIDS, TESTOSTERONE, MUSSELS, FISHES |
