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(16-18) Anaerobic Degradation of Aromatic Amines at Various Temperature Conditions. Russkova, Julia*,1, Kaluyzhnyi, Sergey2, Netrusov, Alexander1, Nekrasova, Valeria3, Lettinga, Gatze4, 1 Department of Microbiology, Moscow State University named after M.V. Lomonosov, Moscow, Russia, Russia2 Department of Enzymology, Moscow State University named after M.V. Lomonosov, Moscow, Russia3 Institute of Microbiology, Russian Academy of Science, prospekt 60-letya Oktyabrya, 7, k.2, Moscow, Russia4 Sub-Department of Environmental Technology, Wageningen University, The Netherlands, Wageningen, The Netherlands ABSTRACT- Pollution of natural and man-made ecosystems with N-substituted aromatic compounds (products of pharmaceutical, dyes, polymers, pesticides, explosives producing industries) raises the problem of the ability to self-remediation. The possibility of anaerobic biodegradation by 4 types of methanogenic consortia (sludge) of aromatic amines (4- and 5-aminosalicylic acids (ASA) and 2-, 3-, 4-aminobenzoic acids (ABA)) was studied, since the limiting step of anaerobic mineralization of any N-aromatic compounds is usually the degradation of aromatic amines. The results suggest that the definition of aminoaromatic compounds, as toxic to methanogenic consortium has to be revised. The aminoaromatics tested practically did not inhibit methanogenesis up to concentrations of 3-5 g/l; moreover, some of them (2-ABA, 4-ABA and 5-ASA) even exert a stimulating effect on aceticlastic activity of the sludge. All sludges completely mineralized 5-ASA and 2-ABA at high (55 oC) and quite low (20 oC) temperatures, however, 4-ASA was not degraded by any of the sludges. 3-ABA and 4-ABA were quantitatively mineralized by one type of sludges at 30 oC. Bioaugmentation by naturally adapted methanogenic consortium to 5-ASA could potentially increase the anaerobic biodegradation of 5-ASA even at concentration 0.1% of adapted sludge added to the non-adapted. The work on the isolation of the pure cultures of 5-ASA anaerobic degrading bacteria is in progress. Key words: aminoaromatics, anaerobic, biodegradation, methanogenic |
