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(46-15) Polybrominated diphenyl ethers (PBDEs) in bream and eel from the river Elbe, Germany. Karasyova, T.1, Sawal, G.1, Stachel, B.2, Lepom, P.*,1, 1 Federal Environmental Agency, Berlin, Germany2 Arbeitsgemeinschaft fuer die Reinhaltung der Elbe, Hamburg, Germany ABSTRACT- Bream (Abramis abrama L.) and eel (Anguilla anguilla L.) samples were collected during a fish species survey in the river Elbe upstream the city of Dresden in April and October 2001. Muscle tissue was isolated and frozen until analysis for PBDEs. BDE congeners 28, 47, 66, 85, 99, 100, 138, 153, 154, 183 and 209 were measured after drying of the muscle tissue with sodium sulphate, extraction with toluene and clean-up of the obtained lipid fraction by gel permeation chromatography and multi-layer column chromatography (silica gel, alkaline- and acid-impregnated silica gel). Quantification of individual congeners was performed by capillary gas chromatography-electron capture negative ionisation mass spectrometry (GC-ECNI-MS) in the selected ion monitoring mode using the BDEs 77, 119, 181 never found in environmental samples and 13C-labelled BDE-209 as internal standards. For congeners with three to seven bromine atoms the most prominent ions at m/z = 79 and m/z = 81 were recorded while for the decabromodiphenyl ether BDE-209 the highly specific ions at m/z = 484,7 and m/z= 486,7 were used for quantification. Results on peak pattern, tissue concentrations as well as a comparison with data on PBDE in fish from other European river systems will be presented. Key words: brominated flame retardants , polybrominated diphenyl ethers, freshwater fish, analysis |
