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(41-28) Free radical by two red tide algae and the damages to gill epithelial cells. Liu, Wenhua*,1, Jack, Ralph, Lam, Paul1, Wu, Rudolf1, 1 Department of Biology & Chemistry, City University of Hong Kong, Hong Kong, Hong Kong, Hong Kong ABSTRACT- Liu W.H. Jack R.J. Lam P.K.S. Wu R.S.S. Key Words: Chattonella marina, Heterosigma akashiwo , reative oxygen species, Na+/K+ATPase, gill epithelial primary cell, lipid peroxidation Presenter: W.H. Liu, City University of Hong Kong Author to contact: Wenhua Liu City University of Hong Kong Department of Biology & Chemistry Hong Kong T:852-21942561 F:852-21942563 wenhua_l@hotmail.com Preference: Platform, but will accept poster 1st Choice: 2A 2nd Choice: 4N I want to be considered for the Young Scientist Award and will be below 30 years of age at the time of the meeting: No Free radical by two red tide algae and the damages to gill epithelial cells Liu W.H.1, Jack R.J.1, Lam P.K.S.1 and Wu R.S.S.1. 1Department of Biology & Chemistry, City University of Hong Kong, Hong Kong SAR. There has been a global increase in the frequency and severity of harmful algal blooms (HABs) in the last two decades, and HABs have often caused massive fish kills and considerable economic loss in many countries. Chattonella marina and Heterosigma akashiwo are two of the major toxic algae which have caused massive fish kills in Asia. However, detail toxic mechanism involved in these fish kills is not well known. Out study demonstrated that these two algal species could generate a high concentration of reactive oxygen species (ROS), such as superoxide anion (O2-), hydrogen peroxide (H2O2) and hydroxyl radical (.OH), which may be responsible for fish kills. We also show that Chattonella marina generates more ROS than Heterosigma akashiwo , and ROS production in both species varies with different growth phases. Primary cultures of mangrove snapper (Lutjanus argentiamaculatus) gill epithelial cells were exposed to crude extract of these two toxic algae, and the effects of ROS produced on Na+/K+ATPase and lipid peroxidation (LPO) were investigated. Our results showed that ROS generated by Chattonella marina and Heterosigma akashiwo may disrupt Na+/K+ATPase activities and cause serious damages to fish gills. This in turn, may lead to asphyxia and fish mortality. Key words: Chattonella marina and Heterosigma akashiwo , reative oxygen species, Na+/K+ATPase, gill epithelial primary cell |
