|
(18-81) Concentration of di(2-ethylhexyl)phthalate in blood derivatives stored in PVC bags. Díaz-Ferrero, Jordi*,1, Pérez, Marta1, Broto, Francesc1, Comellas, Lluís1, Rodríguez-Larena, Ma Carmen1, Argelagués, Enric2, Hernández, José Manuel2, Sauleda, Silvia2, 1 Institut Químic de Sarrià (URL), Barcelona, Spain2 Centre de Transfusió i Banc de Teixits, Servei Català de la Salut, Barcelona, Spain ABSTRACT- Di(2-ethylhexyl)phthalate (DEHP) is a widely spread plasticizer for PVC. Some toxic effects have been described in the literature, such as estrogenic activity and carcinogenicity due to the exposition of laboratory animals to this compound. However, toxicity studies in humans are still scarce. Since DEHP is used as plasticizer in medical material, is highly lipophilic and is not chemically bound to the polymer, there is a human subpopulation with a high risk of exposition to this compound. In this work, we have determined DEHP concentration in plasma, platelet and red-cell samples of 30 volunteer donors. In addition, 6 samples of thalasemic patients, who have received blood transfusion for more than 20 years, have been analyzed. Extraction, fractionation and storage of blood and blood derivatives have been carried out following the usual protocols in Centre de Transfusio i Banc de Teixits. Analytical procedure for DEHP determination consists of a protein denaturation followed by a liquid-liquid extraction and instrumental analysis by HRGC-MS. In plasma samples, the concentrations of DEHP ranged from 11.9 to 22.9 ug/ml; in platelet samples ranged from 7.25 to 19.1 ug/ml and, in red cell samples, from 6.34 to 125 ug/ml. In thalasemic patient samples, DEHP concentration was below the limit of detection. Our results show that blood transfusions can be a significative exposition way to DEHP for the patients. However, this compound is not accumulated in human plasma. Key words: DEHP, PVC, blood derivatives, phthalates |
