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(79-04) Evaluation and further development of the activated sludge respiration inhibition test. Gendig, Cornelia1, Agnoli, Francesca2, Bettiol, Cinzia2, Pagga, Udo3, Strotmann, Uwe*,1, 1 FH Gelsenkirchen University of Applied Sciences, Gelsenkirchen, Germany2 University of Venice, Venezia, Italy3 BASF Aktiengesellschaft, Ludwigshafen, Germany ABSTRACT- Respiration inhibition tests with activated sludge bacteria are of great importance for gaining information about the toxicity of chemical compounds. The toxicity data obtained from these test methods are valuable for assessing ecotoxicological effects of chemical compounds on bacteria and, together with results from other test systems for risk assessment purposes. Toxicity data of chlorinated phenols were determined by the OECD activated sludge respiration inhibition test (OECD, 1993) with an incubation time of 0.5 hour or 3 hours and were verified by four different statistical procedures. The different statistical procedures were critically compared and evaluated. Moreover, there is a serious demand for test systems with a prolonged incubation time to determine the long-term effects of chemicals on bacterial respiration. For this purpose a long-term test system was developed. As a reference substance 3.5-dichlorophenol was used to evaluate this long-term test. Furthermore, these data were compared with data from short-term activated sludge respiration inhibition tests and from submitochondrial particle assays. Key words: respiration inhibition test, bacterial toxicity, submitochondrial particle assay |
