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1A - Environmental analytical methods (MOP/14) Localization of heavy metals on the surface of Vaccinium spp. growing in polluted forests. Bialonska, Dobroslawa1, Zobel, Alicja2, Tylko, Grzegorz3, Kuras, Mieczyslaw4, 1 Institute of Environmental Sciences, Jagiellonian Univeristy, Krakow, Malopolska, Poland2 Department of Chemistry, Trent Univeristy, Peterborough, Ontario, Canada3 Institute of Zoology, Jagiellonian Univerity, Krakow, Malopolska, Poland4 Institute of Plant Physiology, Warsaw Univerity, Warsaw, Mazowieckie, Poland ABSTRACT- We investigated four forest sites directly influenced by different kind of emission from industrial region located wast of Krakow (former part of Silesia known as one of the most contaiminated places in Europe). Two Vaccinium species were chosen for our study; one evergreen (V. vitis-idaea) and the second looses leaves every year (V. myrtillus). We compared amounts of heavy metals located on the leaf surface using AAS analysis, after previous removal of surface wax layer by dipping leaves in almost boiling water (Zobel and Brown 1988). Using scanning microscope (SEM) with EDS we localized elements on the surface histologically. Some of compounds could be located on the surface by deposition from the air, and some as the result of active extrusion to the surface. Upper and lower epidermis differed in amount and structres of deposites. SEM reviled the differences in the localiztion on the area of epidermis along the vain and between the stoma and neighbour cells of ground epidermis. We also found that element levels differed in areas where hyphae of the fungi grew and the neighbour epidermal cells, pointing to importance of histological localization before biochemical analysis of the whole leaf. 1. Zobel A., Brown S., 1988: Determination of furanocoumarins on the leaf surface of Ruta graveolens with an improved extraction technic. J. Nat. Prod. 51:941-946 Key words: plant leaf surface, heavy metals , scanning electron microscopy, histological localization |
