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 PARENT SESSION
2C - Biomarkers
Poster Hall
8:30 AM - Tuesday, 29 April 2003
Chair: Hansen, P.D.1, 1
Co-chair: Vindimian, E.2, 2
(TUP/91) Adverse effects of heavy metal contamination from the Guadiamar River after a mining spill (SW, Spain): Biomarkers, Bioaccumulation and Histopathology.
DelValls, T.A.1, Blasco, J.2, Tuberty, S.R.3, McKenney, C.L. Jr.3, González de Canales, M.L.1, Sales, D.1, 1 Facultad de Ciencias del Mar y Ambientales. Universidad de Cádiz, Puerto Real, Cádiz, Spain2 Instituto Superior de Investigaciones Científicas (CSIC), Puerto Real, Cádiz, Spain3 U.S. Environmental Protection Agency. NHEERL. Gulf Ecology Division, Gulf Breeze, Florida, U.S.A.
ABSTRACT- The heavy metal contamination after the Aznalcóllar mining spill was assessed simulating environmental dissolved concentrations of Cadmium and Zinc. Males and females of red swamp crayfish Procambarus clarkii was chosen as a representative specie in the river and were exposed in the laboratory to heavy metal concentrations of Cadmium (10 and 30  g⋅L-1) and Zinc (1000 and 3000 g⋅L-1) during 21 days. These ranges of concentrations were determined at the Guadiamar River after the accident. During the assay different biomarkers were studied for contamination assessment. Hemolymph samples were taken in females every seven days for vitellogenin/vitellin concentration analysis. At the end of the bioassays animals were dissected and samples of gill, hepatopancreas and muscle tissues were taken for metal bioaccumulation determination. The metallotionein concentration was also measured in hepatopancreas tissues. Histopathological study was also developed in gill, hepatopancreas, muscle, and ovary tissues. Hepatopancreatic and ovarian indexes were studied too. A general validation and comparison of the different biomarkers in P.clarkii was performed. Significance different metal bioaccumulation was found in different tissues and for different metal exposures. It was also shown different biomarker responses for different treatments comparing with controls
Key words: Heavy metals, Aznalcóllar mining spill, Biomarkers, Procambarus clarkii
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