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2N - Field Studies (MOP/138) Ecotoxicological evaluation of SPOLANA industrial plant in Czech Republic based on SPMD sampling. Kočí, Vladimír1, Ocelka, Tomá2, Kochánková, Lucie1, vagr, Andrej1, Grabic, Roman2, Cimlerová, Marta1, Mlejnek, Martin1, árka, Crhová2, 1 Department of Environmental Chemistry, ICT Prague, Prague, Praha, Czech Republic2 National Health Institute, Frýdek-Místek, Ostravsky kraj, Czech Republic ABSTRACT- An unexpected flood in August 2002 caused a serious damage to Czech landscape along Vltava and Labe River watershed especially. As usual, by rivers huge industrial plants are operated. One of the most serious big industrial plants impacted by the flood was SPOLANA in Neratovice, Czech Republic. In former decades various persistent organic substances were produced here and thus released into surrounded soil layers. As the underground water has aroused up a danger of tripping of POPs was serious. This poster describes an application of passive sampling with semipermeable membrane devices (SPMDs) used for ecotoxicology monitoring in the area of SPOLANA industrial plant and in surrounding region. These kinds of sampling were used: air SPMD samplers in an area of SPOLANA plant; water SPMD samplers in Labe River, one up and two down the stream, and on the border with Germany, where Labe River leave Czech Republic, more then this Vltava River were sampled up the confluence with Labe River; and soil samples from the area of SPOLANA plant and selected sites in surroundings. SPMD extract were evaluated with these bioassays: bioluminescent bacterium Vibrio fischeri, chlorococal alga Scenedesmus subspicatus and crustacean Daphnia magna. Soil samples were extracted in methanol and consequently tested with Vibrio fischeri. Tests with dehydrogenase activity were provided, too. Although some toxic response was obtained no serious toxic effects were observed. Selected sites were sampling for soil and the same toxicity tests of methanol extracts of these soil samples were provided. Toxicity test proved to be a good tool to rank samples with different level of contamination. Key words: SPMD, Passive sampling, toxicity-testing, POPs |
