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4B - Human health and environmental connections (WEP/193) Effects of ROFA and Buenos Aires (BA) Air Pollution Particles In Young and Aged Rat Lung Cells. Tasat, Deborah1, 2, Ostachuk, Agustin1, Martin, Susana1, Evelson, Pablo3, Mandalunis, Patricia 3, Dawidowski, Laura2, 1 National University of General San Martin, Buenos Aires, Buenos Aires, Argentina2 National Atomic Energy Commission, Buenos Aires, Buenos Aires, Argentina3 University of Buenos Aires, Buenos Aires, Buenos Aires, Argentina ABSTRACT- Exposure to a high particle burden may alter homeostasis of the lung inducing changes in fluid balance and host defense. In the present study the interaction of ROFA and BA particles with both young (1-2 mo. old) and aged (9-12 mo.old) bronchoalveolar lavage cells (BAL) from Wistar rats was investigated using endpoints of antioxidant-oxidant generation and apoptosis. Various concentrations (0-200 ug/ml) of BA and ROFA particle soluble fractions, were added to BAL cells from young and aged animals, incubated in RPMI-1640 at 37°C for 2hr, 4hr or 18 hr and evaluated for antioxidant enzymes by the TRAP method, for superoxide anion (O2-) by the reduction of nitroblue tetrazolium (NBT) and for apoptosis. ROFA and BA particles showed, even at the lowest dose assayed (10 ug/ml), the ability to generate reactive oxygen species to the same extent as the known inducing agent, TPA, in both young and aged populations. This increase in O2- may cause oxidative stress and may have harmful implications. However, this imbalance was only able to induce apoptosis in the aged population while the young population failed to show morphological or biochemical features resembling those of cell death. We conclude that the impact of these particles on oxidant radical generation and apoptosis is clearly dependent on the maturational state of the animal. Key words: Buenos Aires, air particulate matter, oxidative stress, apoptosis |
