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2C - Biomarkers (TUP/84) THE FEASIBILITY OF ACHE, GST AND CAT AS BIOMARKER OF BIVALVIA CONTAMINATION WITH CHEMICALS IN THE GULF OF GDANSK. Justyna, Kopecka1, Janusz, Pempkowiak1, 1 Institute of Oceanology PAS, Sopot, woj.pomorskie, Poland ABSTRACT- Traditionally, in order to monitor the effect of contaminants on biota, analyses of chemicals contents in organisms are carried out. Recently biomarkers were recognized as useful tool for assessing the pollution impact on marine organisms. This is due to their sensitivity, low cost and specifity. In 2001 and 2002 two species of bivalvia (Macoma baltica, Mytilus trossulus) were collected from several locations in the Bay of Gdansk differing in contamination (Mechelinki contaminated, Sopot clean). Activities of three biomarkers were analysed in foot of Macoma, gills of Mytilus for AChE and CAT, and digestive gland of both species for GST. The lowest activity of AChE (AChE units: nmol/min.mg protein) in the analysed tissues of Mytilus trossulus in the coastal station near Mechelinki were observed (15-20 nmol/min.mg protein). The highest activity of AChE, in selected tissues of Mytilus trossulus was odserved in station GG3 Sopot (ca 38 nmol/min.mg protein). In Macoma baltica collected from ship route the highest activity of AChE in whole tissue was measured (80-83 nmol/min.mg protein). The lowest activity of CAT (CAT units: mmol/min.mg protein) in the mussels was observed in Mytilus from reference site (14 mmol/min.mg protein) and in Macoma from station GG2 mouth of Vistula river (6 mmol/min.mg protein). The same trend was found in this organisms for GST activity (GST units: umol/min.mg protein). The highest activity of CAT and GST were observed in Mytilus from GG3 sation Sopot (CAT: 20 mmol/min.mg protein; GST: 0,20 umol/min.mg protein) , and in MAcoma from GG1 station Mechelinki (CAT: 10 mmol/min.mg protein; GST: 0,45 umol/min.mg protein. Key words: BIVALVES (MACOMA BALTICA, MYTILUS TROSSULUS), BIOMARKERS, POLLUTANTS, GULF OF GDANSK, BALTIC SEA |
