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2Q - Online Biomonitoring (WEP/159) Assays with Daphnia magna and Danio rerio as alert systems in aquatic toxicology. Martins, Jose1, 2, Olivateles, Luis1, 2, Vasconcelos, Vitor1, 2, 1 CIIMAR- Marine and Environmental Research Center, Porto, Portugal, Portugal2 Dep. Zoology and Anthropology- Faculty of Sciences-Porto Univ., Porto, Portugal, Portugal ABSTRACT- The aim of this work was to evaluate the use of ecotoxicity assays with Daphnia magna and Danio rerio as alert systems in water contaminated with toxic substances. For this purpose, a wide bibliographic search was made. The chemical substances and the elements were classified into different chemical categories, according to chemical functional groups. In order to evaluate wether these toxicity assays were suitable for predicting contamination in water for human use, graphics with the LC50 values for D. magna and D. rerio were made using STATISTICA©. The relationships between the different categories of carcinogenic risk and the reference dose for chronic oral exposure (RfD) versus the toxicity for both species (measured as LC50) were also stablished. The use of ecotoxicity assays in alert systems with D. magna seems to be more interesting than those with D. rerio. This is due, not only to the greater sensibility of D. magna to most water contaminants but also to the shorter assay time necessary for this species (24 h) when compared to D. rerio (48-96 h). D. magna assays can be mainly used as alert systems for contaminants as aromatic amines, elemental metals, organochlorine pesticides, chlorinated hydrocarbons, styrenes, other pesticides, inorganic metal compounds, halogenated salicylanilides, chemical asphyxiants, fungicides, carbamates and organophosphates. D. rerio assays can be used as an alert system for organophosphate pesticides, elemental metals, carbamate insecticides, organochlorine pesticides and fungicides. The ecotoxicity assays can also give useful information on carcinogenic risk and on effects of human chronic oral exposure. Key words: Daphnia magna, ecotoxicology, Danio rerio, LC50 |
