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2Q - Online Biomonitoring (WEP/162) (Eco)toxicological screening of selected ionic liquids. Mölter, Kerstin1, Stock, Frauke1, Ranke, Johannes1, Bottin-Weber, Ulrike1, Poczobutt, Jana1, Hoffmann, Jens2, Ondruschka, Bernd2, Filser, Juliane1, Jastorff, Bernd1, 1 Centre for Environmental Research and Environmental Technology, University of Bremen, Bremen, Bremen, Germany2 Friedrich-Schiller-University of Jena, Jena, Jena, Germany ABSTRACT- Ionic liquids have recently gained more and more interest within the field of "Green Chemistry" and with respect to future potential industrial applications. The lack of vapour pressure and their great versatility of chemical and physical properties might allow for the specific replacement of conventional molecular solvents or for the well-directed use as media for bio-catalytic reactions. Despite the broad and divers research into ionic liquids toxicological and eco-toxicological aspects of these compounds hardly had been considered so far. Following the principles of "Green Chemistry", generated substances should possess little or no toxicity to human health and the environment. To obtain first information about the possible (eco)toxicological potential of selected ionic compounds we used three screening methods which are part of the "Test battery ecotoxicity/toxicity" within the Centre for Environmental Research and Environmental Technology (UFT). The luminescent bacteria acute toxicity test with Vibrio fischeri as well as cell viability assays using two mammalian cell lines (promyelocytic leukaemia rat cell line IPC-81 and rat glioma cell line C6) were applied. The tested substances covered a range of different structural modifications of imidazolium ionic liquids to allow a systematic evaluation of effects on the selected biological activities. All three test systems showed increasing toxicity (expressed as EC50 values) due to elongated n-alkyl chain length in R1- as well as in R2-position and these structure-activity relationships could be well described by linear regression analysis. The choice of anion showed no systematic influence and all three biological methods used proved to be suitable for a first screening of ionic liquids. Key words: ionic liquids, ecotoxicity, vibrio fischeri, WST assay |
