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3D - Exposure and effects of environmental contaminants in marine organisms (TU7/5) Effects of two North Sea crude oils on reproduction in Cyprinodon variegatus.. Bechmann, Renee1, Taban, Ingrid1, Jonsson, Grete1, ABSTRACT- The effects of chronic exposure to two types of dispersed North Sea oil (NS1 and NS2) on the reproduction of the model fish Cyprinodon variegatus were tested in two separate experiments in a continuous flow system. In the first experiment the oil dispersion was made by an ultrasonic processor (NS1, mean droplet size 3 micrometer), in the second experiment by a mixing valve (NS2, mean droplet size 12 micrometer). Bodyburden of PAH, PAH metabolites in the bile, survival, and egg production of the adult fish was monitored in both experiments. Hatching success, and survival through first feeding for continuously exposed larvae was observed in the experiment with NS1, and the same parameters for eggs transferred to clean water was studied in the experiment with NS2. The egg production, the hatching success, and the survival of larvae was reduced with increasing oil concentration in the experiment with the NS1 oil (0.1, 0.9 and 5.6 mg/L oil). No negative effects on egg production was, however, observed for fish exposed to 0.1, 0.4 and 0.7 mg/L of the NS2 oil, but eggs from fish exposed to 0.7 mg/L NS2 oil had lower hatching success, and the larvae had lower survival than the control larvae. Fish exposed to 0.4 mg/L NS2 produced more eggs than the control fish. Increased egg production at low exposure concentrations has been observed in previous experiments with C. variegatus exposed to PAH compounds. Different types of oil, and different droplet size of the oil may explain the difference in response. Key words: Cyprinodon variegatus , North Sea crude oil, reproduction, biomarkers |
ysæd, Kjell Birger1, Sanni, Steinar1, Plisson-Saune, Stephan2, 1 RF-Akvamiljø, Stavanger, Norway2 TotalFinaElf Norge AS, Stavanger, Norway, Norway