PARENT SESSION

2C - Biomarkers
Poster Hall
8:30 AM - Tuesday, 29 April 2003
Chair: Hansen, P.D.¹, ¹
Co-chair: Vindimian, E.², ²

(TUP/92) Impact of UV-screens on the early development and stress protein response of zebrafish (Danio rerio).

Wengert, Melanie¹, Schlumpf, Margret², Koehler, Heinz-R.¹, Triebskorn, Rita^{1, 3}, ¹ Animal Physiological Ecology University of Tuebingen, Tuebingen, Germany² Institute of Pharmacology, University of Zuerich, Switzerland³ Steinbeis-Transfer Center for Ecotoxicology and Ecophysiology, Rottenburg, Germany

ABSTRACT- Due to their lipophilic character, UV-screens, like MBC,OMZ, BP-3 or HMS, have a strong potential to bioaccumulate in the environment . Nowadays, UV-screens are not only found in sun milks, but also in a series of other cosmetics. The growing awareness of man to protect skin from sun in order to prevent skin cancer leads to a rising production of these substances. As a consequence, it can be assumed that concentrations of UV-screens in surface waters will increase in the future due to insufficient clearance capacities of municipal sewage treatment plants, particularly in the vicinity of companies producing cosmetic products. Consequences of this for aquatic ecosystems are far from being understood. Up to now, several studies demonstrated high concentrations of UV-screens (MBC,OMZ, BP-3, HMS) in fish tissue. Other studies showed the potential of these substances to mimic estrogens. In the present study we investigated the impact of UV-screens on the early development and the stress protein response of zebrafish embryos. Freshly fertilized eggs of Danio rerio were exposed several concentrations of the UV-Screen 3-bezyliden camphor (3-BC). These concentrations were: 33 ng/L, 100 ng/L, 330 ng/L and 1000 ng/L. In parallel, the respective solvent controls and a water control with reconstituted water were run. For two weeks, we observed the following endpoints: coagulation, epibolie, gastrulation, tail formation, heart rate, eye pigmentation and time of hatching. Furthermore, we took samples (immediately after hatching and at the end of the exposure) for the analysis of heat shock proteins (hsp70 and hsp90). For all parameters, concentration-effect relationships will be presented.

Key words: ELS-test, zebrafish, UV-screens, biomarker