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2F - Site Specific Testing (MOP/107) Development of biological test systems using organisms native to acidic mining lakes. Pickl, Christina1, 2, Moser, Heidrun1, Fomin, Anette2, 1 OekoTox Ltd., Stuttgart, Germany, Germany2 University of Hohenheim, Stuttgart, Germany, Germany ABSTRACT- The environmental monitoring of surface waters in former mining areas can not be done by the use of common biological test systems. The waters are often too acidic or ferrous and a lack of nutrients anticipates the use of common fish or algae test organisms. The aim of the project is the development of new test systems with organisms native to mining lakes, which allows the detection of pollutants under the chemical conditions of mining lake waters. For these investigations different cultures of phyto- and zoo-plankton were screened for their suitability as test organisms, e.g. green algae like Chlamydomonads. or flagellates like Euglena. Some of the required criteria for the new test systems are: easy culture handling, fast reaction under stress, and computer-based detection of the endpoints like growth, motility or form. The influence of the culture conditions like nutrient supply, carbon source or temperature and light regime are investigated with the objective of establishing a standard test method. At first the sensitivity of the new test systems are checked with reference substance, followed by the evaluation of the test system with lake water samples. The first results for some green algae native to mining lakes and other green algae held under similar conditions in culture and test exposure are shown. Key words: acidic mining lakes, bioassay, plankton, image analysis |
