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3B - Marine toxicity evaluation: current practice and applications (MOP/168) Pollution Assessment in Galician coast using an integrating analytical chemistry and sea-urchin embryogenesis bioassay. Saco-Álvarez, Liliana 1, Fernández, Nuria1, Beiras, Ricardo1, 1 University of Vigo, Vigo, Pontevedra, Spain ABSTRACT- To characterize the quality of sediments at key sites in Ria de Pontevedra, sediment samples and wild mussels were collected concurrently for chemical analysis and sea-urchin embryogenesis sediment elutriate bioassays. The chemical analysis consisted of the measurement of the concentration of pollutants in sediment, due to the sediments are, in addition to a reservoir for pollutants, a source of toxicants for marine animals; and the bioaccumulation of pollutants in wild mussels (Mytillus galloprovincialis). The analysis of chemical compounds in tissues of sessile filter-feeding organisms is employed in routine studies of marine pollution since it provides a time-integrated pattern of the bioavailable fraction of pollutants, and often in higher concentrations than those present in water or sediments, facilitating its determination. The Paracentrotus lividus sea-urchin embryogenesis bioassay using sediment elutriated consisted of the incubation of fertilized eggs in sediment elutriate at 20°C for 48 h and the measurement of the embryogenesis success, evaluated as percentage of plutei larvae, and larval length as the biological responses. Intertidal sediment elutriates were obtained according to methods previously described (Beiras and His, 1995; Beiras, 2002). Data were analysed using non-linear multidimensional scaling to obtain an ordination of sampling sites according to the degree of pollution. Key words: Paracentrotus lividus, Bioassay, Mytilus galloprovincialis, Ria de Pontevedra |
