PARENT SESSION

2C (2) - Biomarkers
Hall 7
8:30 AM - 10:15 AM, Wednesday, 30 April 2003
Chair: Hansen, P.D.¹, ¹
Co-chair: Vindimian, E.², ²

(WE7/1) Investigations on a possible correlation of catecholamines in the blood plasma and PCDD/F concentration in the liver of silvercarp (Hypophthalmichthys molitrix).

Theimer, Sandra¹, Jaser, Wolfgang¹, Severin, Gabriele¹, Kettrup, Antonius², Schramm, Karl-Werner¹, ¹ GSF-National Research Center for Environment and Health, Neuherberg, Bavaria, Germany² Technical University of Munich, Weihenstephan, Bavaria, Germany

ABSTRACT- Catecholamines, such as epinephrine, norepinephrine and dopamine can regulate physiological processes. They are produced in the cortex, the adrenergic nervous system and the brain of vertebrates as a response to big effort, hypoxy and stress. Sudden changes of physical parameters in the environment (temperature, current, salinity), and water pollution (chemicals, heavy metals and acidity) can cause stress in fish. The presence of catecholamines in blood in relationship with accumulation of PCDD/F in the liver of silvercarp Hypophthalmichthys molitrix from the Ya-Er Lake (China) was proved. The preparation was done with Sample Clean Up Columns, generally used in the clinical diagnostic. The detection of norepinephrine, epinephrine and dopamine was analysed with High-Performance-Liquid-Chromatography. In this study correlations between catecholamines in the blood and levels of PCDD/F in the liver are shown.

Key words: Hypophthalmichthys molitrix , catecholamines, HPLC