|
1J - Bioaccumulation (WEP/59) Predicting uptake and bioavailability of organic contaminants in terrestrial organisms (Eisenia andrei, Aporrectodea caliginosa) using SPME in different soils. van der Wal, Leon1, 4, Jager, Tjalling2, Fleuren, Roel3, van Gestel, Cornelis4, Hermens, Joop1, 1 Institute for Risk Assessment Sciences (Iras), Utrecht University, Utrecht, the Netherlands, the Netherlands4 Institute of Ecological Science, Vrije Universiteit, Amsterdam, the Netherlands, the Netherlands2 Department of Theoretical Biology, Vrije Universiteit, Amsterdam, the Netherlands, the Netherlands3 National Institute of Public Health and the Environment, Laboratory for Ecotoxicology, Bilthoven, the Netherlands, the Netherlands ABSTRACT- The influence of "bioavailability" on the effects of soil pollution is of great importance in determining adverse effects in contaminated areas. Our work focusses on the applicability of Solid Phase Micro Extraction (SPME) as a tool to estimate bioavailability and internal concentrations of contaminants in terrestrial organisms such as enchytraeids and earthworms. Earlier results indicated the applicability of SPME fibers for this purpose in natural and artificial OECD soil for organic chemicals with a log Kow up to 6. Recently, two different earthworm species were used in bioassays (E. andrei and A. caliginosa) to investigate the accumulation of hexachlorobenzene, PCBs, telodrin and dieldrin from two polluted (i.e. amended with dredge sludge material) soils, and a control soil. Accumulation curves for different pollutants were established both in biota and SPME fibers and equilibrium concentrations were compared and are interpreted in terms of bioavailability of these contaminants. Key words: soil, SPME, bioavailability, terrestrial organisms |
