PARENT SESSION

1A - Environmental analytical methods
Poster Hall
8:30 AM - Monday, 28 April 2003
Chair: Schäffer, A.¹, ¹
Co-chair: Bester, K.², ²

(MOP/2) One-step cleanup for PAH residue analysis from plant matrices using size exclusion chromatography.

Popp, Peter², Wenzel, Klaus-Dieter¹, Engewald, Werner³, Schüürmann, Gerrit¹, ² UFZ Centre for Environmental Research Leipzig-Halle, Department of Analytical Chemist, Leipzig, Germany, Germany¹ UFZ Centre for Environmental Research Leipzig-Halle, Department of Chemical Ecotoxicology, Leipzig, Germany, Germany³ 3University of Leipzig, Faculty of Chemistry and Mineralogy, Institute of Analytical Chemistry, Leipzig, Germany, Germany

ABSTRACT- The higher extraction efficiency using the ASE technique requires in many cases also a significant improvement of the quality of the previous cleanup procedure. A new one-step cleanup procedure is described based on size exclusion chromatography (SEC). The method is suitable for the determination of polycyclic aromatic hydrocarbons (PAHs), especially from complex plant matrices (e.g. pine needles, needles, leaves, mosses). The main improvement compared to conventional procedures is that substance peaks are hardly overlapping in the chromatograms, and that it is a very rapid and simple one-step procedure with improved analytical quality. Essential advantages of this SEC procedure are the sharper GC-MS chromatograms for the PAH fraction at retention times between 9.2 and 12.0 min, distinctly separated substance peaks resulting in higher analysis values, shorter run times, and lower solvent consumption.

Key words: size exclusion chromatography, cleanup, PAHs, pine needle