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2L - Immunotoxicity - genotoxicity - ED (WEP/115) Optimisation, automation and application of bioassays for fast quality assessment of soils. Filzek, Petra1, Dott, Wolfgang1, Eisentraeger, Adolf1, 1 RWTH Aachen, Institute of Hygiene and Environmental Medicine, Aachen, Germany ABSTRACT- The water extractable ecotoxic and genotoxic potential of contaminated soils can be detected using bioassays like the Vibrio fischeri luminescence inhibition test (DIN-EN-ISO-11348 T1-3, 1999) and the umu-genotoxicity test (DIN 38415 T3, 1996; ISO 13829, 2000). Round robin tests with different contaminated soil samples have successfully been carried out using these and other bioassays. Nowadays it is well accepted that bioassays are useful supplements to routine chemical and physical analyses, especially for remediation of multi-contaminant sites. Furthermore, they may be a useful tool for the determination of soil sampling strategies. Whereas methods of on-site chemical analyses have already been applied widely before or during remediation in laboratory containers, there is almost no experience with bioassays for on-site analysis. For this reason, the above mentioned bioassays are optimised with respect to sample preparation, test performance and evaluation in order to obtain data in a shortened period of time and to reduce the amount of work by automising the assays. Sensitivity and reproducibility of these assays and the standardised assays are compared by testing reference compounds as well as contaminated soil samples. Based on these results, recommendations concering application and quality assurance for the on-site application of bioassays are given. Possible fields of application are outlined and the benefits of on-site testing are demonstrated. Key words: Contaminated soil, Bioassay, On-site analysis |
