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2N - Field Studies (MOP/126) Development of a test-battery for the assessment of ecotoxic effects in small running waters. Pohl, Bernhard1, Hammers-Wirtz, Monika1, Ratte, Hans Toni1, 1 Aachen University, Department of Biology V, Worringerweg 1, D-52056 Aachen, Germany ABSTRACT- The lowland river Nette (D) is a tributary of the river Niers (D) and belongs to the catchment area of the river Maas (NL). The catchment area (165 km2) is situated in the international natural preserve area Maas-Schwalm-Nette. In the summer the headwater of the stream Nette consists up to 90 percent of effluents dumped by municipal sewage plants. Preliminary investigations showed that these effluents are strongly characterized by chemical compounds which originate from textile industry and agricultural use. The aim of the presented study is to create a suitable test-battery for the assessment of ecotoxic stress in small running waters which are comparable with the Nette. Both water and sediment samples are tested. In particular the results of a population test with Daphnia magna (water phase) will be shown. Other tests investigating the water phase are the duckweed growth inhibition test (Lemna minor) and a test with fish eggs (Danio rerio). The river sediments were assayed with a bacteria test (Arthrobacter globiformis), the chironomid toxicity test (Chironomus riparius) and with a nematode growth and reproduction test (Caenorhabditis elegans). Screening for genotoxicity and estrogenic effects was done with the umu-Test and an estrogen sensitive reportergene cell line (MVLN). Key words: population test, test-battery, genotoxicity, estrogenic effects |
