PARENT SESSION

3B - Marine toxicity evaluation: current practice and applications
Poster Hall
8:30 AM - Monday, 28 April 2003

(MOP/164) Chemical validation of an analytical method for sediment for the ER-Calux and DR-Calux.

Leonards, Pim¹, Lamoree, Marja², Klamer, Hans³, Bakker, Joop³, ¹ Netherlands Institute for Fisheries Research, IJmuiden, The Netherlands, The Netherlands² Institute for Environmental Studies, Amsterdam, The Netherlands, The Netherlands³ National Institute for Coastal and Marine Management, Haren, The Netherlands, The Netherlands

ABSTRACT- An analytical method for the extraction and clean-up of sediment for the ER-Calux and DR-Calux was validated. Recoveries of spike sediment were measured for a large group of contaminants (e.g. nonylphenol, bisphenol-A, atrazine, parathionethyl, naphthalene, benz[a]acridine, DDT, DDE, dieldrin, PBDEs, dioxins, PCBs, etc.) with a broad range of physical-chemical properties. Extraction with Soxhlet was compared to accelerated solvent extraction (ASE) using two types of solvents (hexane:aceton and dichloromethane:aceton). For the ER-Calux cleaning of the sediment extract was performed with gel permeation chromatography (GPC), and for the DR-Calux both with GPC and a multiplayer silica column. In addition, the recoveries of the test compounds for the carry-over step from an organic solvent to DMSO, which is used in the ER- and DR-Calux, were determined. The method was used to quantify the ER-Calux and DR-Calux response of North Sea sediments. Recoveries of the test compounds were in general higher than 70% for the Soxhlet and ASE using DCM:aceton as extraction solvent, except for naphthalene and parathionethyl. The carry-over experiments (organic solvent to DMSO) showed that at high concentrations of the test compounds effects on the availability could be detected only. The DR-Calux response of sediment with the multiplayer silica clean-up was on average 70 times lower than the response of the GPC cleaned extracts. This is probably due to unknown contaminants, for instance unidentified PAH-type compounds with dioxin-type toxicity, and natural substances that can cause a DR-Calux respons.

Key words: ER-Calux, Chemical validation, DR-Calux, TIE