PARENT SESSION

4D - Veterinary Medecine
Hall 13
1:45 PM - 3:30 PM, Monday, 28 April 2003
Chair: Montforts, M.¹, ¹
Co-chair: Boxall, a;², ²

(MO13/14) Residue and Effect assesments of antibacterial agents in two different soils - a field-scale study.

Jacobsen, Anne-Marie¹, Halling-Sørensen, Bent¹, Jensen, John², Sengeløv, Gitte³, Vaclavik, Elvira¹, Ingerslev, Flemming¹, ¹ The Royal Danish School of Pharmacy, Copenhagen, Denmark, Denmark² Danish Veterinary Institute, Copenhagen, Denmark, Denmark³ National Environmental Research Institute, Silkeborg, Jutland, Denmark

ABSTRACT- Residue and Effect assesments of Antibacterial agents in two Different Soils – A Field-scale Study. Jacobsen A.M.1, Halling-Sørensen B.1, Jensen J.2, Sengeløv G.3, Vaclavik E.1 and Ingerslev1 1The Royal Danish School of Pharmacy, Copenhagen, Denmark. 2National Environmental Research Institute, Silkeborg, Denmark. 3Danish Veterinary Institute, Copenhagen, Denmark. There is a basic lack of information concerning accumulation of residues of antibacterial agents in agricultural soils from year to year, as well as antibiotic resistant genes from pathogenic bacteria. The purpose of this study was – over time – to follow the fate of Chlortetracycline (CTC), Sulphanilamide (SUL) and Tylosin A (TYL A) on two agricultural fields located in Southern Denmark, a sandy loam soil (soil A) and a sandy soil (soil B). Three replicate plots on each field were applied with manure containing the three antibacterial agents and further fortified to a total content in soil of approximately 30-100 g/kg and the fields were seeded with spring spring barley. Soil samples were collected from each plot for chemical analysis and detection of antibiotic resistance on 6 occasions during a 152-day experimental period from May to October 2000, and grains were harvested in august 2000. CTC and TYL were, contrary to SUL, measured throughout the entire sampling period, with starting concentrations of approximately 30 and 50 g/kg declining to approximately 1 and 5 g/kg, respectively, by the end of the experiment. Half-lives for soil A and B were estimated for CTC to 25 and 34 days, respectively and for TYL A to 67 and 49 days, respectively. There was no significant difference between soil types. Barley grains were analysed for antibiotic content, and very low concentrations of CTC were measured. The effects on soil fauna community and antibiotic resistance levels were assessed in relation to application of manure containing antibacterial agents to the agricultural fields.

Key words: antibacterial agents, Veterinary medicine, fate, arable land