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2A - Mixture toxicity (MOP/66) Mutagenicity study of landfill leachate extracts by Ames test. Shu, Hung-Yee1, Wu, Yuh-Shen1, Chang, Ming-Chin1, 1 Hungkuang Institute of Technology, Shalu, Taichung 433, Taiwan, Taiwan ABSTRACT- In Taiwan, landfill was the most popular solid waste disposal method. However, the landfill leachate may cause further environmental pollution problems. The biological treatment was traditionally employed to deal with landfill leachate before discharging into surface water body. Frequently, the leachate property (water quality and quantity) varied significantly so that the biological treatment operation was not effective. Therefore, the effluent from the biological treatment failed to comply with the national discharge standards frequently. Thus, it may damage the environment and human health. In order to identify the mutagenic effect of leachate, the extracts from solid phase extraction (SPE) procedure and total solids (TS) were collected for Ames Salmonella assay. The mutagenicity of landfill leachate was examined with Salmonella Typhimurium TA98 and TA100 in presence or absence of S9 mix. The SPE extracts of leachate samples from three landfill sites showed the significant direct and indirect mutagenicity with strain TA98 and TA100. However, one year later, the same landfill leachate was collected from municipal solid waste landfill with age from 10 to 15 years and tested again. The impact of direct and indirect acting frameshift mutagens from leachate extracts decreased significantly. Furthermore, leachate from landfill with 3 to 4 years landfill age and received both municipal and incinerator ash, showed highly mutagenic to strain TA98 and TA100 without metabolic activation. Therefore, the result implied the presence of some specific direct acting frameshift mutagens. Key words: leachate, landfill, mutagenicity, Ames test |
