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1H a/b/c - Pesticides, pharmaceuticals, perfluoroalkylated substances, antibiotics (WEP/50) Genotoxicity of Three Pesticides Using the Computerized Analysis of DNA Content and Cell Cycle Analysis in Mouse Bone Marrow. El-Khatib, El-Hussein1, 1 Department of Mammalian Toxicology,Central Agricultural Pesticides Laboratory, Agriculture Research Center, Dokki., Giza., Giza, Egypt ABSTRACT- This study was carried out to assess the genotoixicity of diazinon, organophosphate; aldicarb (Temik)Carbamate; and cyfluthrin (Baythroid),pyrethroid; using the computerized image analysis in mouse bone marrow cells. Two oral dose levels of each tested pesticide were used in the present study as follows: diazinon: 2 and 10 mg/ kg b.w.; aldicarb: 0.01 and 0.05 mg/kg b.w.; and cyfluthrin: 20 and 60 mg/kg b.w. Animals were killed 24 hours after receiving single oral dose. The obtained data indicated that the two major effects of these pesticides are the reduction in the proportion of cells having 4c-DNA content and the accumulation in cells having 2c-DNA content. The automatic cell phase classification indicate clearly a progressing and significant decrease in the cell proportion in GO/ G1 for the increasing the pesticide doses, and the percentage of cells in the phase was significantly decreased. The mode of action of these pesticides may be due to G1-phase arrest in bone marrow cells, causing damage or alteration to DNA synthesis. Key words: Genotoxicity, Pesticides, Cell Cycle Analysis , DNA Content |
