PARENT SESSION

1B+C Abiotic Transformations + Biodegradation
Poster Hall
8:30 AM - Monday, 28 April 2003
Chair: Peijnenburg, W.¹, ¹
Co-chair: Jafvert, C.T.², Gombar, V.K.³, ^{2 3}

(MOP/24) Characterization of Bacterial Community in Waste Landfill by Terminal-Restriction Fragment Length Polymorphism Analysis.

ISHIGAKI, TOMONORI¹, LEE, TAE HO², YAMADA, MASATO¹, KAMAGATA, YOICHI², ¹ NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES, Tsukuba, Ibaraki, JAPAN² NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, Tsukuba, Ibaraki, JAPAN

ABSTRACT- Various chemicals that exist in waste landfills are degraded or transformed chemically and biologically. Although microorganisms should be expected to play very important role in fate of chemicals, microbial community in waste landfill has been paid no attention and has been unknown well. Attempts were made to elucidate the structure of microbial community in waste landfill for better understanding of chemical fate. Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis was applied to analysis of eubacterial community in leachate of waste landfill. The profiles of T-RF from different community of landfill leachate were completely different. Phylogenic groups of each T-RF were determined by comparing with clonal library of 16S rRNA genes. T-RFs were affiliated with the Alcaligenes, Pseudomonas, Sphingomonas and clones belonging proteobacteria epsilon and delta subdivisions. Furthermore leachates enriched by several chemicals were subjected to T-RFLP analysis, and functional role of the community member that was affiliated to each T-RFs was discussed by the succession of bacterial community.

Key words: microbial community, waste landfill, 16S rRNA gene, Terminal-Restriction Fragment Length Polymorphism