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2N - Field Studies (MOP/139) Application of SPMD technology for monitoring of Czech rivers pollution. Hajslova, J.1, Kocourek, V.1, Setkova, L.1, Kazda, R.1, Suchan, P.1, 1 Institute of Chemical Technology, Prague, Czech Republic ABSTRACT- Semipermeable membrane devices (SPMDs) have become a valuable tool for obtaining information on bioavailability and distribution of hydrophobic organic pollutants in particular environmental compartments. Contrary to grab sampling based on direct measurement (SPE, L-L extraction etc.) reflecting residue composition at the moment of sampling, the integrative sampling strategy employing SPMDs does not fail to detect episodic contamination. SPMDs, in general, concentrate all neutral chemicals having molecular masses < 600 Da. As regards aquatic ecosystem, the sampling of fish or other biota commonly used as bioindicators of water pollution may be substituted. Experiments concerned with modification of existing isolation procedure used for processing of exposed SPMDs were carried out within the part of project aimed at improvement of analytical methodology. Instead of time-consuming isolation step employing dialysis for recovering of sequestered target compounds, extraction of cut membranes by hexane enhanced by sonication was employed. Separation of trioleine from analytes by gel permeation chromatography followed. Better / comparable recoveries of sequestred chemicals could be obtained in this way, in addition, compared to "classic" approach (dialysis), the sample handling process prior to the determinative step was significantly reduced. To demonstrate the character of pollution, comprehensive 2D GC TOF (time of flight) MS technique was applied. Excellent resolution of sample components together with identification potential of this system provided a complex information on the characteristic pollution pattern in individual sampling sites. Monitoring program focusing on pollution of main Czech rivers (Elbe, Moldau) by SPMDs was established in 1997. PAHs, PCBs and related organochlorine compounds, polybrominated flame retardants (BFR), musk compounds and alkylphenols represent the main groups of chemicals involved in our study. The comparison of pollution patterns before and after floods that occurred in Czech Republic in August 2002 will be documented. This study was carried out within the project No. MSM 223300004 supported by the Ministry of Education and Youth of the Czech Republic. Key words: SPMD, monitoring programme, Czech rivers pollution |
