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Jong Kim1 *, Sampath Parthasarathy1
Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA 1
Glycodelin, also known as placental protein 14 (PP14) or pregnancy related endometrial protein, is a glycoprotein produced from epithelial glands of human endometrium. Low serum glycodelin levels in habitual abortors and its function in inhibiting natural killer cell activity suggest its role in implantation or lack of it in fetal loss. Our goal is to study the role of glycodelin in pregnant mouse model. The objective of this study was to determine the presence of glycodelin in the mouse. Using an anti-peptide polyclonal antibody to human glycodelin sequence, which recognized human glycodelin by immunohistochemistry and by western blotting, we determined the presence of mouse glycodelin. In this study, proteins from mouse amniotic fluid and serum, and isolated uterine proteins were separated by gel electrophoresis. We detected 45kDa protein by western blotting corresponding to glycodelin from human amniotic fluid. Then immunohistochemistry was performed using cryostat sections from non-pregnant and pregnant (day 7 and 15) mouse uterus. This antibody immunostained endometrial epithelial glands of non-pregnant mouse uterus as in human. In day 7 pregnant mouse uterus, visceral yolk sac was strongly immunostained. In day 15, this antibody strongly immunostained mouse visceral yolk sac and placenta was also stained. This is the first study demonstrating the presence of immunoreactive glycodelin in the mouse. Immunoreactive glycodelin during pregnancy suggests its possible role in supporting implantation or in protecting fetus via immunosuppressive function, which can now be studied using mouse model.
This abstract is being presented on Monday, August 2 at 8:00 AM to 10:15 AM at CUB 2nd Floor Ballroom.